Composite

Part:BBa_K3196024

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)


AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.
Figure1. T--HUST--China--2019-PHO1-αproVP


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None